ABSTRACT
<p><b>OBJECTIVE</b>To establish standard serum proteomic pattern for early liver cancer and explore its value in early liver cancer diagnosis.</p><p><b>METHODS</b>The serum samples were obtained from 12 healthy subjects and 12 patients with early liver cancer. After removal of serum albumin and IgG, 300 µg serum sample was mixed with the hydrating solution for two-dimensional gel electrophoresis. Each experiment was repeated for 3 times. With silver staining and image analysis, the differentially expressed proteins were identified to establish the standard proteomic pattern for early liver cancer. The proteomics pattern was then tested in the diagnosis of 13 cases of chronic liver disease and 10 healthy individuals in a double-blind manner.</p><p><b>RESULTS</b>Thirty-eight differentially expressed protein spots were identified. Eleven protein spots with differential expressions by over 5 folds were analyzed to establish the standard proteomics pattern for early liver cancer, which showed a sensitivity of 92% and specificity of 90% in early diagnosis of liver cancer.</p><p><b>CONCLUSION</b>The standard serum 2-DE proteomic pattern for early liver cancer shows distinct differences from that of healthy subjects. The differentially expressed proteins and their specific combination pattern may have the potential for diagnosis and prognostic evaluation of early liver cancer.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Biomarkers, Tumor , Blood , Blood Proteins , Early Detection of Cancer , Methods , Electrophoresis, Gel, Two-Dimensional , Methods , Liver Neoplasms , Blood , Diagnosis , Proteome , Metabolism , Proteomics , MethodsABSTRACT
<p><b>OBJECTIVE</b>To investigate the infection of Helicobacter pylori (Hp) in the liver tissue of patients with chronic liver disease and the association between Hp and chronic liver disease.</p><p><b>METHODS</b>Liver tissue samples were obtained by liver biopsy and surgical resection from 30 healthy subjects, 30 patients with chronic hepatitis, 30 with cirrhosis and 30 with liver cancer. All the samples were confirmed by pathological examination. The gene fragment coding for 16SrRNA were amplified by PCR with sequence analysis.</p><p><b>RESULTS</b>The PCR product of the 16SrRNA gene was 109 bp in length. Hp 16SrRNA was detected in 18 out of 30 liver biopsy samples from patients with primary cancer (60.0%), in 14 samples from patients with liver cirrhosis (47.0%), and in none of the samples from normal subjects or patients with chronic hepatitis. Sequencing analysis of Hp 16SrRNA gene in the liver tissue showed a 98.8% homology with the gene fragment encoding Hp 16SrRNA.</p><p><b>CONCLUSION</b>Hp is identified in the liver tissue of patients with chronic liver disease, suggesting the possible correlation between Hp infection and hepatocellular carcinoma.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Base Sequence , Helicobacter Infections , Diagnosis , Microbiology , Helicobacter pylori , Genetics , Hepatitis, Chronic , Microbiology , Liver Cirrhosis , Microbiology , Liver Neoplasms , Microbiology , Molecular Sequence Data , RNA, Bacterial , RNA, Ribosomal, 16SABSTRACT
<p><b>OBJECTIVE</b>To survey the status of Helicobacter pylori (Hp) infection in the liver of patients with liver cancer and explore the correlation between Hp infection and liver cancer.</p><p><b>METHODS</b>Liver specimens were obtained 30 patients with chronic liver disease (CLD), 30 with liver cancer, and 30 healthy control subjects. The specimens were cultured in Colombian agar medium in microaerophilic condition for isolation of Hp. The presence of Hp was also identified by biochemical test, urease test, immunohistochemistry, and PCR and sequence analysis.</p><p><b>RESULTS</b>Hp was found in none of the liver specimens by culture tests. One out of the 30 patients with liver cancer (3%) was positive for Hp by urease tests. Immunohistochemical staining identified Hp positivity in 9 liver cancer tissue (30.0%), but in none of control or CLD cases (P<0.05). PCR detected Hp 16S rRNA in 18 out of the 30 patients with liver cirrhosis (60.0%) and in none of the control or chronic hepatitis cases. Sequencing analysis of Hp 16S rRNA in liver tissue showed a 98.8% homology with the Hp16S rRNA gene.</p><p><b>CONCLUSION</b>A greater incidence of Hp infection in the liver is identified in patients with liver cancer than in healthy subjects and CLD patients, suggesting a potential correlation between Hp infection to liver cancer.</p>